Journal:

Article Title: Long Term Synaptic Depression That Is Associated with GluR1 Dephosphorylation but Not ?-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor Internalization *

doi: 10.1074/jbc.M803431200

Figure Lengend Snippet: Neither GluR1 nor GluR2/3 total surface expression is changed 45 min following cLTD treatment, as determined by cross-linking assay. A, representative blots of X-linked (25.0 μg of total protein loaded) and un-X-linked (5.0 μg of total protein loaded) samples for control and cLTD conditions probed with antibodies specific for GluR1 (left group) and GluR2/3 (right group). B, representative blots of un-X-linked and X-linked (5.0μg of total protein loaded for both) samples probed with antibodies specific for PP2Aα (top bands) and synapsin (bottom bands). C and D, graphs of cumulative data comparing X-linked control and X-linked cLTD samples probed with antibodies specific for GluR1 (C) and GluR2/3 (D)(n = 8). E and F, data from C and D normalized to the transferrin receptor. G and H, graphs of cumulative data comparing the percent surface expression (see “Experimental Procedures” for explanation of calculation) between control and cLTD conditions for GluR1 (G) and GluR2/3 (H)(n = 8). I and J, graphs of cumulative data comparing un-X-linked and X-linked samples probed with antibodies specific for PP2Aα (I) and synapsin (J)(n = 8). No significant differences were observed for any of the comparisons as determined by Student's paired t test. Values represent the mean ± S.E.

Article Snippet: Primary antibodies were as follows: GluR1 and GluR2/3 (Chemicon) used at 1:3000 in BSA, phospho-Ser-845 and phospho-Ser-831 (PhosphoSolutions) used at 1:1000 in BSA, PP2Aα (BD Pharmingen) used at 1:5000 in milk, synapsin, NR2B, and NR1 (PhosphoSolutions) used at 1:2000 in BSA, the α subunit of calcium/calmodulin-dependent protein kinase II (αCaMKII) (BD Pharmingen) used at 1:3000 in BSA, phospho-Thr-286 (PhosphoSolutions) used at 1:3000 in milk, PSD-95 (Affinity Bioreagents) used at 1:5000 in BSA, and transferrin receptor (Zymed Laboratories Inc.) used at 1:1000 in milk.

Techniques: Expressing, Control

Journal: International journal of molecular sciences

Article Title: Treadmill Exercise Alleviates Cognition Disorder by Activating the FNDC5: Dual Role of Integrin αV/β5 in Parkinson's Disease.

doi: 10.3390/ijms24097830

Figure Lengend Snippet: Figure 6. Effect of treadmill exercise on synaptic plasticity of hippocampal pyramidal neurons and dopamine levels and BDNF in MPTP-induced chronic PD mice model. (A) Total dopamine levels of the hippocampus (F(4,55) = 33.12, p < 0.001, n = 12). (B) Hippocampal protein levels of PSD95, synapsin, synaptophysin, SNAP47, TH, and BDNF were examined by Western blotting. (C–H) Quantification of PSD95 (F(4,10) = 53.50, p < 0.001), synapsin (F(4,10) = 99.18, p < 0.001), SNAP47 (F(4,10) = 60.12, p < 0.001), synaptophysin (F(4,10) = 17.47, p < 0.001), TH (F(4,10) = 83.36, p < 0.001),and BDNF (F(4,10) = 215.7, p < 0.001) levels. (n = 3). (I) Ultrastructure of synapses determined via electron micrography in hippocampus CA1 region of mice (scale bar = 500 nm). Enlarged images of the left images (scale bar = 125 nm). Red arrows indicate postsynaptic densities (PSDs), green arrows indicate the width of the synaptic cleft, blue arrows indicate the thickness of the PSD, and red dotted lines indicate the length of active synaptic zone. (J–L) Quantification of the length of the active synaptic zone (F(2,6) = 264.3, p < 0.001), synaptic cleft width (F(2,6) = 23.87, p = 0.001), and PSD thickness (F(2,6) = 21.17, p = 0.002), (n = 3). (M,N) Representative section of Golgi-Cox staining dendrites of CA1 pyramidal neurons from the stratum radiatum in mice. Spines number/10 µm (F(2,6) = 11.73, p = 0.009). Scale bars = 10 µm, 25 µm, and 100 µm; n = 3. Data represent the mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control; # p < 0.05, ## p < 0.01, ### p < 0.001 vs.

Article Snippet: These primary antibodies included TH (1:1000, ab112, Abcam, Shanghai, China), PSD95 (1:1000, 3450S, CST, Fall River, MA, USA), Synapsin (1:1000, NB300-104, NOVUS (Shanghai) Co. Ltd, Centennial, CO, USA), CAMKII(1:1000, 50049S, CST, Fall River, MA, USA), SNAP47 (1:1000, ab172609, Abcam, Shanghai, China), Synaptophysin (1:1000, ab52636, Abcam, Shanghai, China), BDNF (1:200, ANT-010, Alomone Labs, Jerusalem, Israel), FNDC5 (1:1000, bs-8486R, Bioss, Beijing, China), integrin αV (1:200, sc-9969, Santa Cruz, Dallas, TX, USA), integrin β5 (1:200, sc398214, Santa Cruz, Dallas, TX, USA), and β-actin (1:5000, 60008-1-Ig, Proteintech, Wuhan, China).

Techniques: Western Blot, Staining, Control